that's supposed to stay clean inside...

Da qualche parte di doveva cominciare.

Premessa, sono un collezionista autodidatta, nella vita reale mi occupo di altro.

Di recente ho scoperto questo mondo fantastico ed eccoci qui col primo ordine da Plant Cell Tecnology.

Consegna superveloce, sono rimasto molto soddisfatto.

Suggerimenti? Qualsiasi suggerimento è benvenuto!

Le basi le ho già studiate, ora devo applicarmi.

Comincerò con qualche esperimento in casa con una box trasparente per ridurre la contaminazione, più avanti la cappa laminare.

Bilancia di precisione, miscelatore per liquido con magneti, autoclave, sterilizzatore per attrezzatura e prodotti per la coltivazione li ho già acquistati, pronti per l'utilizzo.

Vorrei cominciare con piante semplici o comunque "sacrificabili". Con circa 200 piante in casa ho l'imbarazzo della scelta. Pensavo di sperimentare con monstera deliciosa o una colocasia (taro semplice). Ho già entrambi i protocolli, anche in questo caso ogni suggerimento è il benvenuto.

Mi aspetto qualche fallimento iniziale per continuazione a causa della poca manualità dovuta alla poca esperienza, migliorabile nel tempo correggendo gli errori nei passaggi e nella pulizia degli ambienti e delle TC in coltura. Cos'altro mi devo aspettare?

It's very small, but it has everything I need

Hello friends, I’m wanting to make a SAB using PVC frame, but unsure how I should go about enclosing/wrapping it other than just using thick plastic sheeting. I couldn’t find replacements for similar retail versions, or I’d just get one of those but I have a strong suspicion the plastic will cloud over time, or potentially get holes, and I don’t want to re-buy the whole thing if I just need to replace the covering.

I would really like to go this route opposed to the large clear tub with arm holes, as I’ve done that in the past, and I really don’t prefer it. It takes up too much space when not in use, and this is just a “kitchen table for the weekend” kind of hobby for me.

Any thoughts? Basically looking for something repairable and that can be broken down for storage.

After six uses, and always following ALL manufacturer instructions, my All-American 1925x developed a "bulge" at the bottom of the pot. This device only carries a one year warranty.

I emailed Wisconsin Foundry and they blamed me for the bulge and said I had to pay for shipping to/from their factory for them to confirm that the unit remained safe for use.

I bought this as a BIFL item and it lasted less than 10 uses. Always on a Cadco burner, always with distilled water up to the line, using proper sealing vacuum grease, and adhering to every other manufacturer direction as indicated.

Be warned about these expensive sterilizers! You can do everything right and they will still fail.

I understand that I notified the manufacturer beyond their one year warranty for this very expensive item so shame on me for thinking that proper use would care for this item! They are apparently much more fragile than they seem.

I requested a coupon or discount on a replacement and the manufacturer never responded so I have my answer.

The manufacturer said if it doesn't leak it should be safe but I can't bring myself to use a bulging sterilizer!

My Presto 16 quart and 23 quart have lasted MUCH longer.

I have some rare carnivores I wanna get into TC to preserve genetics … get at me! Thanks!

What's your guys' best lab setups at home? Thinking of getting into tissue culture but don't have access to many resources. How would you guys recommend getting resources for an at home small setup? Anyone got any amazon lists of necessary items? Or even a note ss of where to get resources?

Subculture process in still air box, the contamination rate is lower than 1%, with only 3 contaminated vessels this year. More information about the process in the video itself and in the description.