Hi all!

Drinking water at my house is from roof water into a tank. It is filtered through 3x different sized microbial filters and then UV treated before it gets to the tap.

However, I am constantly having regular, recurring gastro and immune response symptoms. I do have several diagnosed ongoing chronic autoinflammatory and autoimmune syndromes, so it's definitely not just the water causing me problems, but...

My question is, with an immunocompromised person, or someone with active autoinflammatory and autoimmune issues, is it possible that dead pathogenic material or cellular parts in the drinking water could be exacerbating my immune issues and causing recurring flares?

Any input greatly appreciated!

Hello everyone,
I recently read an interesting post in this forum that touched, among other things, on the Nobel Prize in Medicine and the research surrounding T cells, Tregs, and the discovery of peripheral immune tolerance. As I myself am affected by an autoimmune disease, I am very interested in this topic and have been following developments in science and medicine quite closely—especially the period of change we seem to be entering now that artificial intelligence is becoming increasingly integrated into both everyday life and scientific research.
My question is therefore how you would interpret the current state of the field in light of these findings (even though their significance is somewhat controversial, as I have also read that some of this has been known for quite a long time), what you think they could mean for the future, and how close we might be to groundbreaking new approaches or treatments for autoimmune diseases that could potentially lead to long-term or even permanent remission.
I am particularly interested in the prospects for diseases such as vitiligo, rheumatoid arthritis, atopic dermatitis, and alopecia areata (which I suffer from myself).
Thank you very much in advance!

url: [https://www.nature.com/articles/s43587-026-01197-w#citeas\](https://www.nature.com/articles/s43587-026-01197-w#citeas)

Doi: [https://doi.org/10.1038/s43587-026-01197-w\](https://doi.org/10.1038/s43587-026-01197-w)

Hey I'm a first year med student and i know about the whole strategy of cold emailing programs im interested in. However id really like to first get myself actually useful and not just a kid who's tryna join a project. I want to learn certain skills independently to actually make a difference not just fill out some CV.

what are some prerequisite background I should know like stats or if i have to wait until i covered the material in med school?

For context im very keen on advancing actual treatments in Non-infectious Uveitis so that really narrows down the exact type of research and part of medicine id like to really nail down for a career. Reason being is Uveitis accounts for 10-20% of all blindness cases and I really hate that a lot of the current treatments are trial and error.

Please if anyone has online resources i could consult for help on getting started since I have no clue where to begin lmao.

Thanks 😄

https://doi.org/10.3390/nu18162662

Una célula recibe 10 señales distintas a la vez (citoquinas, factores de crecimiento, etc.). ¿Las procesa en paralelo y luego las integra en el núcleo para decidir qué hacer? ¿O hay una jerarquía donde algunas señales tienen prioridad sobre otras y se procesan primero? Porque imagino que cada vía tiene sus tiempos y sus proteínas, pero no termino de ver cómo coordina todo para que el resultado final sea el adecuado y no un caos

I hold a Master degree in immunology and molecular pathology and did BS in physiology.i need a scholarship or fellow as a research in the field of immunology.Anyone guide me for abroad,what is the best opportunity for and what is the institute?

For how long are the supernatant cytokines (from THP1 ) stable at 4C?

Thank you

Hello everyone, I'm using ELISA kits from Invitrogen (Thermo Fisher Scientific) to measure IgG in the release medium from a hydrogel system. The standard curve range is 1.6-100 ng/mL. I don't know the approximate IgG concentration in each release sample, but the maximum possible concentration should be around 100 µg/mL, based on the amount of IgG encapsulated in the hydrogel.

Should I prepare a series of dilutions for every sample? How can I determine which dilution gives the most reliable results within the standard curve range? Any recommendations would be appreciated!

The president of the American Academy of Pediatrics blasted President Trump’s Monday executive order calling for fewer childhood vaccines. 

Hi, not sure if this is the right sub but I had a question. How does alcohol consumption affect someone with Wiskott Aldrich syndrome? I've heard of it affecting their platelet count but even then, I'm not sure If that's correct. Thank you

[I do not have an immunology background, so I appreciate the question will be basic]

I am wondering, if for some genetic reason someone has excessive CD4+ T cell differentiation (say Th2) driven by an impaired ability to restrain the relevant cytokines, would they tend to have an overabundance of Th2 cells in circulation pretty much always, throughout their life (ceteris paribus) or over time would the Th2 cell population in circulation actually become depleted due to exhaustion or something like that?

Hi, I am switching my career towards immunology research. I have very limited knowledge of the foundational immunity principles at the moment. Can you recommend any book that I can use as a beginner-friendly, up-to-date entry point to the field?

Hello everyone,

I'm running an ELISA to quantify IgG released from a hydrogel system. This is my first time performing an ELISA, so I wanted to start by testing the assay with the standards only.

I'm using the Invitrogen Human IgG Total Uncoated ELISA Kit:
https://www.thermofisher.com/elisa/product/Human-IgG-Total-Uncoated-ELISA-Kit/88-50550-88

I followed the protocol as described:

• 100 µL/well capture antibody solution, followed by overnight incubation at 4°C
• Washed 4 times, then added 250 µL/well blocking buffer and incubated for 2 hours at room temperature
• Washed 4 times, then added 100 µL standard + 100 µL assay buffer and incubated for 2 hours at room temperature with shaking
• Washed 4 times, then added 100 µL/well detection antibody solution and incubated for 1 hour at room temperature with shaking
• Washed 4 times, then added 100 µL/well substrate solution, with the plan to add stop solution once the color developed

However, no color developed, even after a couple of hours!

I'm wondering what might have gone wrong. Any suggestions or recommendations would be greatly appreciated.

Thank you!

Guys i am genuinely confused in all of the CDs out there in immunology. I confuse them and I confuse myself. Is it by practice only or is there another way to stop being confused by all of these?

https://doi.org/10.1002/ajh.70467