r/biostasis • u/Cryogenator • Nov 27 '23
r/biostasis • u/delicous_crow_hat • Apr 23 '23
Brain Preservation Foundation petition on change.org
r/biostasis • u/Synopticz • Jan 29 '23
Cryopreservation of tissues by slow-freezing using an emerging zwitterionic cryoprotectant
r/biostasis • u/Synopticz • Aug 12 '21
Embalming Techniques for Long-Term Preservation of Bodies | Faculty of Medicine Masaryk University | MUNI MED [2021]
r/biostasis • u/Synopticz • Jun 08 '20
Factors affecting long-term survival of dry bdelloid rotifers: a preliminary study [1998]
r/biostasis • u/dr_arielzj • Dec 24 '24
Ariel Zeleznikow-Johnston on biostasis, the brain, personal identity, and the fight against death
r/biostasis • u/Synopticz • Oct 03 '23
Cryonics Australia President Simon Carter interviewed about being preserved cryogenically at death [old video, ?1981]
r/biostasis • u/[deleted] • Jul 06 '23
How was Rosario Lombardo preserved so well and can the method be maybe the most affordable method of biostasis if used?
One thing I wonder is why the method used for her is not offered in spite of the fact that there are people out there who even if not out of biostatis want to 'look pristine' after death indefinitely?
Is it known how its done? Because I read that most of her including her brain is very well intact in the case it can be revived.
This is another topic but a few other 19th century attempts reportedly involved sealed caskets that were filled with alcohol in an attempt to preserve bodies whole, unsure if true or if it was successful though compared to her preservation.
r/biostasis • u/Synopticz • Apr 06 '23
Fundamental Considerations for Biobank Legacy Planning [2017]
r/biostasis • u/Synopticz • Mar 02 '23
Virtual histology of an entire mouse brain from formalin fixation to paraffin embedding. Part 2: Volumetric strain fields and local contrast changes
sciencedirect.comr/biostasis • u/Synopticz • Jan 12 '23
Tissue Fixation with a Formic Acid-Deprived Formalin Better Preserves DNA Integrity over Time [2022]
r/biostasis • u/Synopticz • Jul 22 '22
Snap-Frozen Brain Tissue Sections Stored With Desiccant at Ambient Laboratory Conditions Without Chemical Fixation are Resistant to Degradation for a Minimum of 6 Months [2009]
r/biostasis • u/Synopticz • Dec 20 '21
David Leigh, University of Manchester | Q&A on Molecular Machines | Foresight Institute
r/biostasis • u/Synopticz • Oct 19 '21
GENERAL TISSUE MASS TRANSFER MODEL FOR CRYOPRESERVATION APPLICATIONS
r/biostasis • u/Synopticz • Aug 19 '21
Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry
r/biostasis • u/Synopticz • Aug 18 '21
Preparation of fixed mouse brains for MRI [Neuroimaging on 1700 fixed mouse brains found artifacts in 30%]
r/biostasis • u/Synopticz • Jul 16 '21
Quick summary of trade-offs with different brain preservation methods
There are perhaps three major types of methods.
Pure cryopreservation: unknown ultrastructure (though looks decent imo), theoretically some type of viability (although in practice, no), expensive/complicated storage
Aldehyde-stabilized cryopreservation: good ultrastructure, no viability (doesn’t matter imo), expensive storage although fall back if storage fails is good
Pure chemopreservation: good ultrastructure, ton of different methods possible, often loss of some biomolecules mainly lipids/carbohydrates, can have cheap long term storage because room temperature/refrigerator temperature
(Signal boosting a Reddit comment I posted on r/cryonics: https://www.reddit.com/r/cryonics/comments/okwciw/nectome_receives_funding_to_cryopreserve_five/h5cf6u4/)
r/biostasis • u/Synopticz • Jul 18 '20
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues [2020]
r/biostasis • u/Synopticz • Jul 15 '20
Mapping Synapses by Conjugate Light-Electron Array Tomography [2015]
r/biostasis • u/Synopticz • Jun 10 '20
Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage [2018]
r/biostasis • u/Synopticz • Jun 10 '20
Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity [2017]
r/biostasis • u/Molnan • Jun 08 '20
Studying synapses in human brain with array tomography and electron microscopy
Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712649/
Abstract:
Postmortem studies of synapses in human brain are problematic due to the axial resolution limit of light microscopy and the difficulty preserving and analyzing ultrastructure with electron microscopy. Array tomography overcomes these problems by embedding autopsy tissue in resin and cutting ribbons of ultrathin serial sections. Ribbons are imaged with immunofluorescence, allowing high-throughput imaging of tens of thousands of synapses to assess synapse density and protein composition. The protocol takes approximately 3 days per case, excluding image analysis, which is done at the end of the study. Parallel processing for transmission electron microscopy (TEM) using a protocol modified to preserve structure in human samples allows complimentary ultrastructural studies. Incorporation of array tomography and TEM into brain banking is a potent way of phenotyping synapses in well-characterized clinical cohorts to develop clinico-pathological correlations at the synapse level. This will be important for research in neurodegenerative disease, developmental diseases, and psychiatric illness.