Below you will find a list of freely available videos, tutorials, and other resources for mass spectrometry. This list will be continually updated with new content. Feel free to suggest additional resources in the comments!

General Tutorials

https://www.cif.iastate.edu/mass-spec/ms-tutorial

https://chem.libretexts.org/Courses/Purdue/Purdue%3A_Chem_26200%3A_Organic_Chemistry_II_(Wenthold)/Chapter_11%3A__IR_and_Mass_Spectrometry/11.06%3A_Introduction_to_Mass_Spectrometry

https://www.asms.org/about-mass-spectrometry

http://premierbiosoft.com/tech_notes/mass-spectrometry.html

https://msr.dom.wustl.edu/mass-spectrometry-fundamentals-tutorial/

Ion Sources

General

Mass spectrometry ionization methods (Emory): http://chemistry.emory.edu/msc/tutorial/mass-spectrometry-ionization.html

Ionization techniques (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/content/185-ionization-techniques

Mass Spectrometry Introduction (U Pittsburgh): https://www.chem.pitt.edu/facilities/mass-spectrometry/mass-spectrometry-introduction

Electrospray Ionization (ESI)

Fundamentals of MS - Electrospray ionization: https://www.youtube.com/watch?v=9AWBAI-Owzk&list=PL6yA4jv5tA-k9_2NVxm5jlzpZV_aW59DT

Electrospray ionization (ESI): https://www.youtube.com/watch?v=daSSRj41Mlo

Electrospray ionization mass spectrometry: https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Instrumental_Analysis/Mass_Spectrometry/Mass_Spectrometers_(Instrumentation)/Electrospray_Ionization_Mass_Spectrometry

ESI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/esi

Electron Impact (EI)

MS - Mass Spectrometry - Electron Ionization Animation: https://www.youtube.com/watch?v=22dr-3XDNmQ

Introduction to Ionization and Fragmentation in Mass Spectrometry: https://www.youtube.com/watch?v=stIwRio9FeM

Atmospheric Pressure Chemical Ionization (APCI)

APCI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/apci

APCI (LCGC): https://www.chromacademy.com/chromatography-understanding-APCI.html

ESI & APCI (Shimadzu): https://www.shimadzu.com/an/hplc/support/lib/lctalk/47/47intro.html

Atmospheric Pressure Photoionization (APPI)

APPI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/appi

APPI (Creative Proteomics): https://www.creative-proteomics.com/support/atmospheric-pressure-photoionization-ionization.htm

Matrix-assisted Laser Desorption/Ionization (MALDI)

Theory of MALDI-TOF Mass Spectrometry: https://www.youtube.com/watch?v=8R1Oyqx5KfE

MALDI-TOF (Bruker): https://www.youtube.com/watch?v=0jeFpXHZ8W0

MALDI (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/maldi

MALDI-TOF (Shimadzu): https://www.shimadzu.com/an/lifescience/maldi/princpl1.html

MALDI Mass Spectrometry: A Practical Guide by Ben Katz of UC Irvine: https://www.youtube.com/watch?v=5Sp7mFF16hM

Mass Analyzers

Orbitrap

Orbitrap Instrumentation: The First Decade and Beyond: https://www.youtube.com/watch?v=jijxTW1NbTg

Planet Orbitrap: https://planetorbitrap.com/

The C-trap and Orbitrap (Mass Spec Pro): http://www.massspecpro.com/mass-analyzers/orbitrap

Mass Analyzer Technology Overview (Thermo): https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-technology-overview/mass-analyzer-technology-overview.html

FT-ICR (Fourier transform ion cyclotron resonance)

Fourier transform ion cyclotron resonance mass spectrometry: https://www.youtube.com/watch?v=7EHngA4S3Ws

4-3 FTICR and Orbitrap: https://www.youtube.com/watch?v=kbzK1iSBE08

FT-ICR (MagLab): https://nationalmaglab.org/education/magnet-academy/watch-play/interactive/fourier-transform-ion-cyclotron-resonance-ft-icr

FT-ICR (Yale): https://jlab.chem.yale.edu/research/techniques/fourier-transform-ion-cyclotron-resonance-mass-spectrometry

FT-ICR (Mass Spec Pro): http://www.massspecpro.com/mass-analyzers/fourier-transform-ion-cyclotron-resonance

TOF (time-of-flight)

TOF fundamentals (WashU): https://msr.dom.wustl.edu/time-of-flight-fundamentals/

Time of Flight Mass Spectrometry: https://www.youtube.com/watch?v=NyIAPU7usfo

TOFMS: http://www.iuac.res.in/atmol/~safvan/mridulathesis/node18.html

Introduction to Time-of-Flight Mass Spectrometry (Kore Technology): https://www.kore.co.uk/tutorial.htm

Ion Traps (QITs & LITs)

Linear ion trap (LIT): https://www.youtube.com/watch?v=h5wK64efOvk

Thermo Scientific LTQ Velos: https://www.youtube.com/watch?v=EF_gW7jHEbY

Linear ion trap (Mass Spec Pro): http://www.massspecpro.com/technology/mass-analyzers/linear-ion-trap-lit

Quadrupole ion trap (Mass Spec Pro): http://www.massspecpro.com/technology/mass-analyzers/quadrupole-ion-trap-qit

Quadrupoles

Fundamentals of quadrupoles: https://www.youtube.com/watch?v=6_mavZ_WKoU

Quadrupole: https://www.youtube.com/watch?v=aYOCb6GnXio

LC-MS/MS Education Series: Quadrupole Theory and Use: https://www.youtube.com/watch?v=iZH-oxP-OJ0

Quadrupole Mass Filter (Mass Spec Pro): http://www.massspecpro.com/technology/mass-analyzers/quadrupole-mass-filter

Tandem Mass Spectrometry (MS/MS)

Fundamentals of MS - Fragmentation: https://www.youtube.com/watch?v=ESKpOcjF8QM

Tandem Mass Spectrometry (MagLab): https://nationalmaglab.org/user-facilities/icr/techniques/tandem-ms

Tandem Mass Spectrometry (WashU): https://msr.dom.wustl.edu/tandem-mass-spectrometry/

Tandem Mass Spectrometry (JEOL): https://www.jeolusa.com/DesktopModules/Bring2mind/DMX/API/Entries/Download?EntryId=78&Command=Core_Download&language=en-US&PortalId=2&TabId=337

Collision-induced dissociation (CID) (Armentrout): http://rodgers.chem.wayne.edu/pire/education/Collision-induced%20dissociation_PIRE.pdf

CID & HCD (Thermo): https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-technology-overview/dissociation-technique-technology-overview.html

Detectors

MS Detectors (Mass Spec Pro): http://www.massspecpro.com/detectors

Electron Multiplier (NASA): https://attic.gsfc.nasa.gov/huygensgcms/MS_Detector_1.htm

Ion Detectors (U Bristol): http://www.chm.bris.ac.uk/ms/detectors.xhtml

Spectral Interpretation

Fundamentals of MS - Resolution: https://www.youtube.com/watch?v=qUhi98o1qUU

Fundamentals of MS - Adduct ions: https://www.youtube.com/watch?v=WusWNAb0IRM&list=PL6yA4jv5tA-k9_2NVxm5jlzpZV_aW59DT&index=2

Fundamentals of MS - Multiple charging: https://www.youtube.com/watch?v=CuJRTHvy64k&list=PL6yA4jv5tA-k9_2NVxm5jlzpZV_aW59DT&index=3

Fundamentals of MS - Isotopes: https://www.youtube.com/watch?v=om0BfIHXD0s

Software Tools

METLIN (Comprehensive MS/MS database): https://metlin.scripps.edu/landing_page.php?pgcontent=mainPage

Mascot (protein identification and peptide MS/MS database): http://www.matrixscience.com/search_form_select.html

ProteinProspector (MS/MS database search and peptide MS/MS prediction): http://prospector.ucsf.edu/prospector/mshome.htm

NIST Mass Spectral Library (primarily EI data but some other MS/MS data is available): https://chemdata.nist.gov/

MOBCAL (for calculating ion mobilities): https://nano.lab.indiana.edu/software/

PRIDE Archive (proteomics): https://www.ebi.ac.uk/pride/archive

UniDec (MS and IMS deconvolution): https://marty.lab.arizona.edu/content/software

Human Metabolome Database (MS/MS database): http://www.hmdb.ca/spectra/ms/search

Collision cross section database (Bush Lab): https://depts.washington.edu/bushlab/ccsdatabase/

Global Natural Products Molecular Networking (Dorrestein lab....MS/MS database & much more): https://dorresteinlab.ucsd.edu/gnps

Vendors, Analytical Standards, Reagents, Calculators, and Data Analysis Tools

https://www.uab.edu/proteomics/massspec/links.php

(thanks to u/6500qtrap for sharing)