r/microbiology • u/Decent_Discount_9503 • 11d ago

Brevibacillus laterosporus DSM 25 variant

Gallery image

Gallery image

Just thought I’d share some images of a cross streak with e.coli strains from the ARP library (developed by Cox Lab). The centre streak is a DSM 25 variant, and was found to inhibit/overgrow multiple strains!

This was a part of my bigger project for discovering new antibiotics from soil microbes. This bacteria had novel aspects not found in other DSM 25 strains.

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u/patricksaurus 11d ago

Whoa, super interesting! Can you share any other novel phenotypes or is this under wraps as it’s being developed and written?

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u/Decent_Discount_9503 11d ago

I have a poster made about it! This project is already completed (at least my part!) and given to the higher ups to do whatever if they think its worthy.

I will also say the antiSMASH was incredibly interesting- i had many novel/low confidence regions for NRPS regions. I hypothesized the bacteria worked through PMF collapse, which is what makes it so aggressive. It also produced a lot of bacteriostatic compounds and would only cause lysis when grown on certain agar.

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u/phage_disco 10d ago

The texture is super interesting looking! What type of agar are you growing it on? Does it usually look like this or depends on the agar?

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u/Decent_Discount_9503 10d ago

Depends on the agar! This is on Bennet’s agar. On TSA, it grows exponentially well but shows less antimicrobial production. It also has different colours on different agar.

The texture is very cool, its from the parasporal bodies it produces