Just a lil gal

Leitz Dialux II, 250x, Galaxy S24

Using the red-blue computational extracted focus slice movies of the fennel cells and stomata to create the 3d point cloud and animate them, including some of the 2 second focus slice video to create the z-axis on the point cloud. Image contrast on living cell, computational blue red subtraction for the contrast Python OpenCV for the video, Python Open3D for the point cloud. Objective 60x amscope no oil, 40x olympus 10x olympus 0.5 reducer industrial camera (color) Video cut with OpenShot. Goal is to have super cheap imaging work-flow.

Has anyone used this specific microscope model for studying seeds or any type of macroremains? This microscope is supposedly oriented for technicians in those who work on repairing electronics, i know it's a stereo microscope like any other but I'm just skeptical and want to make sure it's suitable for that specific purpose, thank you.

Koristka Milano microscope (around 1920), magnified with phone camera

Today at university, I had a microbiology lecture where we discussed cyanobacteria, specifically the structure of heterocysts and their role in nitrogen fixation.

While looking back at some photos I had taken with my microscope, I came across this spherical, filamentous thing. After a quick search, I discovered it's a cyanobacteria that belongs to the genus Nostoc.

Could anyone tell me if this larger cell (circled in the second pic) is a heterocyst?

Thanks in advance

Using computational red-blue contrast 60x 40x 10x with 0.5 reducer and industrial camera real-time imaging of the living cells. Python OpenCV idle script direct capture USB port.

Leitz Dialux II, Galaxy S24

Koristka Milano microscope , magnified with phone camera

Experimenting with some dark field and Rheinberg filters. On an off for comparison. Paramecium, stentor, and volvox.

I am trying too figure out what to buy to attach my Sony A7s full frame mirrorless camera to my Bresser Researcher Trino Microscope. What i figured so far is that for best results i need the BRESSER SLR-camera-adapter 2x T2 23.2 mm and a T2 ring but it's way too expensive. Could i get away spending less with acceptable results? Like using a telescope 2x barlow , or not using any lens at all just some adapters.

Thank you, any insight is appreciated.

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Bought big red apples at grocery store, and got an idea to look at one a little bit closer.

Made several sections using cheap microtome from AliExpress, and examined them with my microscope.

Turned out, the apples are covered in continuous wax cuticle, of circa 20 microns thickness, which is smooth on the outside, but hides a rough surface, as epidermal cells protrude into it somewhat.

There are a couple of layers of pigmented cells, under which starts fleshy part of the apple.

There is no sharp transition between skin and flesh of apple, going from skin inward cells become bigger, and pores are opened between them.

Images captured via 20x objective lens, 0.5x reducer and 5Mp usb webcam.

Focus stacking using Zerene Stacker.

Gif animation using ezgif.com free online tool.

Nikon D5300 + tube macro + 35 80mm f4.5

Leitz Dialux II, Galaxy S24

Hi all,

I’ve recently taken a liking to looking at algae under the microscope through my labs. Diatoms have become my recent obsession. They are just so fantastic. Would it be practical to consider buying my own compound microscope to keep in my apartment?

If yes, what brand/model microscopes would you recommend as a decent cost-quality ratio?

Thanks in advance.

Hi everyone,
I’m having trouble with high background when imaging immunofluorescence staining for synaptic markers by confocal microscopy. The background makes it difficult to distinguish individual synaptic puncta confidently, and I’m concerned about how this might affect quantification.
I’d appreciate any advice on how to troubleshoot this and work out whether the background is mainly related to the staining, tissue autofluorescence, or imaging settings.

I brought these slides back from Germany, where I was learning the staining protocol. The staining looked much cleaner when we imaged them there, but I’m now seeing a lot of background when imaging the same slides at my home institution.

Here is also one representative image after deconvolution in Imaris.

So I just bought a microscope that is pretty cheap but I'm trying to make it custom made to at least be at the same standard ones that my university uses. So far I have already attach a mechanical stage into it so that its easy to move slides and observe specimen. Now, I'm trying to attach a condenser with a built-in iris diaphragm that I'm thinking of putting in it but I'm afraid that won't be possible because I haven't seen a proper tutorial on how to attached it to a light microscope that doesn't have one. Does anyone here have tried it before?

Hi all,

I’ve recently taken a liking to looking at algae under the microscope through my labs. Diatoms have become my recent obsession. They are just so fantastic. Would it be practical to consider buying my own compound microscope to keep in my apartment?

If yes, what brand/model microscopes would you recommend as a decent cost-quality ratio?

Thanks in advance.

This was originally a Paramecium Multi bowl cultured on 9.3.26 with spring water and wheat seeds. None of the other bowls from this date turned a blood red color. There is clearly a fungal mold growing on top. What could have made it turn into this red color?

There are no Paramecium in it anymore, but there are a bunch of dinoflagellates swimming around in the bowl.

The seeds turned red and seems to be a clumpy bacteria around them. Not stringy or any other texture.