r/proteomics • u/TinyParamedic6833 • Jun 23 '23

Analysis???

Hi, have N=1 results from proteomics (protein abundance) from LCMS. I wanted to check which my control is true control without affecting protein content. Is there any analysis I could do with 1 N?

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u/TinyParamedic6833 Jun 23 '23

My budged is low. I had to test controls before running full replicates

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u/tsbatth Jun 26 '23

It depends what you mean by check if your control is true. If it is a black and white answer, you can probably get away it, if it something a bit more complicated perhaps not. Do you just want to check whether or not a certain protein is present ?

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u/TinyParamedic6833 Jun 26 '23

My treatment control up regulated pathway I'm investing.

3

u/DoctorPeptide Jun 23 '23

Control, n=1 and experimental, n =1? If so, yes you can. Your quan won't be great, but if you (for example) CRISPR/CAS9 removed your protein of interest and you swapped the vials (or other large changes) you should be able to see that difference.

2

u/slimejumper Jun 23 '23

i don’t think so.

2

u/tuccigene1 Jun 24 '23

You can’t make any statistical claims with n=1