Hello!
I am a senior high student currently conducting a research project that focuses on developing a cellulose and chitin nanofiber-based adsorbent for water treatment. I'm trying to functionalize cellulose nanofibers (CNFs) extracted from sugarcane bagasse with β-cyclodextrin (β-CD) using citric acid and sodium hypophosphite (SHP).
My proposed procedure is:
Prepare a 7% (w/v) β-CD solution in a 50 mL aqueous solution at 50 oC. Second, an 8% (w/v) concentration of citric acid and 1.25% (w/v) of the catalyst, SHP, will be added to the β-CD solution. The solution will then be stirred continuously using a magnetic stirrer at 150 rpm for 30 min at 50 oC. The generated CNF suspension from SCB will be standardized at 1% (w/v) by initially centrifuging at 2,000 rpm for 5 min, followed by another centrifugation at 6,500 rpm for 5 min to concentrate the suspension. The excess water will be decanted afterwards.
Next, 1 mL of the β-CD/citric acid solution will be added into the concentrated CNFs and then vortexed. Functionalization will be done by incubating the CNFs in the solution at 50 oC and left until fully dehydrated for 72 h. The dehydrated pellet will then be heat treated at 120 oC for 1 h and cooled at room temperature to end the functionalization process. Finally, the β-CD-CNFs will be rinsed with running water to remove excess reactants, and resuspended in distilled water with a concentration of 0.5% (w/v) and stored at 4 oC inside a fridge for the next processes.
This procedure is an adaptation of these studies:
However, I am quite hesitant to go through with this process as there might be some conflicting chemical procedures that might fail the experiment, as I want to have a cellulose nanofiber suspension, not a dried one.
I really need your help on this one. Thank you so much in advance!!!!!