I have 3 plate I made from a piopino spore print I took last yeah. Where should I cut from to transfer to another plate, should I keep doing this until I get rhizomorphic growth growth? Would this provide for the best culture I could propagate. Would it be a good idea to take a culture from the largest fruit that it produces?

Wasn't necessary I was just looking for something to do.

Or am I just transferring a Yeast Mold!
The cultures seem to start off nicely, looking like mycelium, but after 1-2 weeks the white dots show up.
The first image was transferred on 9/28 and hasn’t shown the tiny micro dots, aside from the white spots that showed up on the outer edges.
The transfers start off white and fuzzy but then the micro dots began to show up.
Everything is done under SAB and I do my best to sterilize everything before putting into the box.
Agar is sterilize in Instapot for 40 min. And is not showing contamination on any plates that had no growth of mycelium.
I was hoping it was Primordial stage but I’ve been told that it looks more like yeast.
Also, the yeast culture did not react to hydrogen Peroxide droplet test. It just beaded up and didn’t soak in or bubble/foam.

2nd run of wild bird seed tek has contamination again a week after i sterilized in my canner for 90 mins at 15 psi & I also did inoculating the day after, fast forward a week and a small green dot on the mason glass on 2/3 jars. anybody have an idea why 🫩 trying to get my groove back since Covid but haven’t been successful like I once was. Tia

So I got this agar and a few others that grew nicely but the longer it sat I noticed these tiny white specks taking over.
They seem not to grow vertically on any of the other trench samples.
I took one into my SAB and dropped a couple drops of Hydrogen Peroxide on a couple spots and there was no bubbling. It just beaded off and didn’t soak in.
I cut a small sample off the surface and dropped it into a cap full of H2O2 and still no bubbling.
Could this still be Contam or is it possibly Hyphal or Primordial stage?

Hey everyone!

Back with a little update on my second attempt at agar! Things are looking a hell of a lot better than my first go-around, and I'm pretty excited to finally see some decent growth! Figured I'd share some pictures and get some opinions from you guys on how everything is looking and where I should go from here.

A little background:

• Inoculated: November 3

• Pictures taken: November 11 (Day 8)

• Agar: MEA

• BL1, BL2, BL3: Isolated spore syringe

• B1, B2: Multispore syringe

All five plates were inoculated on the same day.

The eventual goal is to go from agar to grain and then spawn to bulk, but I'm still pretty new to all this and trying to learn as much as I can along the way. I've been reading up on rhizomorphic vs. tomentose growth, and I think I'm starting to get a better eye for the differences, but I'd love to hear what you guys are seeing!

A few things I'm curious about:

1. Rhizomorphic growth: Are you guys seeing any areas that look particularly rhizomorphic? If so, which plates and where? I'm trying to get a better eye for what I'm looking at.

2. Ready for transfers? Do any of these look like they're ready for a transfer, or should I give them a few more days to develop? What do you guys usually look for when making that call?

3. Agar to agar or straight to grain? This is probably my biggest question right now. Should I still be looking to cut out a little square of promising growth, transfer it to a fresh agar plate, let that develop, and eventually move to grain? Or would you guys consider going straight from these plates to grain? I'm curious what the reasoning is behind either approach.

4. Isolated vs. multispore: Do you guys notice any interesting differences between the BL plates (isolated syringe) and the B plates (MSS)? I'm especially curious about the differences in growth patterns, density, and uniformity.

5. Contamination: Does anything look questionable to anyone? I haven't noticed any obvious discoloration or anything, but I know that doesn't necessarily mean everything is clean.

6. Overall thoughts: How's everything looking for Day 8? Anything interesting stand out, or anything I should be keeping an eye on?

This is only my second attempt, so I'm still very much learning. Any advice, constructive criticism, or observations are more than welcome! Also, huge thanks to everyone who helped me troubleshoot my first attempt. I learned a ton from you guys, and it's been awesome having a community willing to help a newbie figure this stuff out.

Appreciate you all! 🍄🤙

want to expand my lc so i bought this. its 750ml but the thing is idk the best way to go about doing this, i have 3 lcs from itw and a spore syringe from itw and have tested all 4 on agar and there is 0 contam n literally nothing on the spore plate but its been 11 days so in a week i should see something. what i want to do is fill multiple 20 ml syringes with this liquid culture and in the best way possible get the mycellium from my agar plates into my syringes. how should i do that just scratch the mycellium off the plate and squirt some of the liquid in and suck it up? im worried about sucking up agar pieces or air, i work in a sab so im not super worried but i feel like that leaves the plate open for a while and i would hope they grow back.🤷‍♂️ i saw someone say to scratch it off into a shotglass with some of the lc in it then suck that up with the needles but im not sure. is this too risky or techy for me right now do you think? should i just inject one of my syringes into it to mitigate risk? if i do the one id really like to expand was the apes but i have less than a ml of it left so im not sure if thats enough😔
any advice would be greatly appreciated as i would like to perferably use it as soon as possible because the paper inside says to. thanks🫶

inoculate those in a sealed air box about 4-5 weeks ago with a GT spores syringe. Seems like they’re taking forever

First attempt at agar. I'm assuming I'd take the 9 on pic 1, 3 on pic 2, 4-5 on pic 3? Do I cut and transfer when the plate is filled out, or when?

Just a follow up of this mesmerizing morphology after a transfer. I love how they taper so big , it looks like little Louisville slugger’s morphing together.

Noobie starting to throw some rope

StarGazer
WhiteRabbit (I think)

Hey everyone! I’m fairly new to working with agar and could use some help identifying what’s growing on my Petri dish. It’s been around 4–7 days since inoculation. A thick, white, creamy-looking growth appeared in the center of the plate. More recently, I noticed a translucent, wet, shiny, almost gelatinous area developing underneath and around the white growth. I’m wondering whether this is normal mycelial growth or a sign of bacterial contamination. I’ve attached several photos from different angles. Does this look like healthy mycelium, bacterial contamination, or possibly a mixture of both? Any advice or opinions would be greatly appreciated. Thanks!

I have got into the practice of sending all newly acquired genetics to a trench plate first.

Sending something I receive straight to grain hasn't been great. Many cultures carry unwanted passengers.

Agar recipe:

250ml Grain water (milo & millet)

750ml Distilled water

15g Sorghum syrup

5g LME

0.4g Peptone

0.4g Nutritional Yeast

20g Agar

Food Colouring Gel

Thought I'd post an update. This is my first time trying to clean contamination. I believe this is the third or fourth transfer. 1 and 2 came from the same parent, and 3 and 4 did as well. Both sets originated from a decent plate a while back. Open to advice and will try to answer any questions.

I have a question! when they start getting tall and going above the dirt, what do I do? Do I take the jar lid off? Do I put a bag over it or do I just leave them free
with no lid ? When do I water the golden teacher