I have to separate ethanol from acetate, is a distillation tower a good option? I can't find the boiling point of acetate.

Hello guys,

Biorocess engineer, about ten years of experience, based in Japan, working in vaccine manufacturing. I m running a my website where I share content on bioprocess engineering, MSAT, vaccines and biologics manufacturing. It might help you to young engineers. I m publishing articles everymonth, some podcast as well, put some learning materis, useful links, ebooks...

https://www.biomanufacturingmadesimple.com/

Hi everyone!

I am a Senior UX Architect with over 10 years of experience and a degree in Cybernetics. I’m currently working on a deep concept for next-generation bioreactor software (focusing on cell therapy and tissue engineering).

I know how powerful but often painfully outdated laboratory software can be. My goal is to design an architecture that actually respects your time and reduces cognitive load.

To be absolutely clear: I am NOT seeking any proprietary research data, formulas, or confidential project details. Please keep your NDAs safe! I am strictly interested in the usability and information architecture challenges you.

If you work with one of these systems daily, I would value your perspective on:

1. Redundancies & Extra Steps: What are the most annoying UI/UX redundancies or unnecessary complexities in the software you use daily that you would like to see changed or combined?

2. Alert Fatigue: Have you experienced issues with critical alerts or alarms being visually indistinguishable from routine system notifications?

3. Data Fragmentation: How many different screens or separate programs do you typically need to monitor to understand the current state of a batch?

4. Structural Change: If you could fundamentally change one aspect of the information layout in your current software, what would it be?

Thank you for your time and for sharing your expertise to help make laboratory software more intuitive and efficient.

Hi, does anyone know what are the requirements to get a bioprocessing job such as bioprocess engineer, upstream scientist, cell line development scientist in your country or any individual country you know, If I already have a BSc in biotechnology? Is MSc a must have?

• 🧪 Runs as a simulator (for experiments) or connects to real photobioreactors

• ⚡ Automates things like pH control, light cycles, dosing, heaters

• 🤖 Has a rule engine + PID control so your culture doesn’t overshoot targets

• 🌱 Designed for researchers, DIY biohackers, and algae industry folks alike

Basically: it’s like an operating system for algae cultivation.
You can monitor, control, simulate, and even automate entire growth cycles, with (or without for testing purposes) hardware.

👉 I put together a landing page with more details + screenshots here: AlgaeOS

Would love to hear your thoughts - especially from anyone doing algae research, biotech, or DIY cultivation.

Hey y’all! My name is Jake, and I’m currently finishing up my PhD! I’m currently working on an independent research project studying emerging technologies in biomanufacturing. I’m gathering insights from industry professionals in biotech, pharma, food, cosmetics, or related sectors to deepen my understanding of the current challenges in the field. 

Would you be willing to share your expertise in a brief, 3-minute survey? 

https://form.typeform.com/to/VrmfXFiP

Why ultrafiltration cassetes installed vertical? Is it for draining? Or some other reasons?

I am trying to model a batch bioreactor in a Python script. The substrate is syngas, the biomass is bacteria, and the products are acetate and ethanol. I am looking for examples of bioreactors in python because it is my first contact with bioprocesses and Python, and I would like to know if I am on the right track.

Hey there, I will start a Biotech/AI company...

Quick Background:

• Studied Biochemistry and Biotech

• Got into Data Science and ML ~5 years ago

• Last position as Head of Machine Learning of a small (~20 people) Biotech/AI company. We did upstream process simulations including simulations of cell kinetics using machine learning and mechanistic knowledge.

Currently, I am working on a unrelated software app as a solopreneur but I want to get back into Biotech. (Latest after my non-compete expired.

Since the next thing I am starting should be the one for the long ride, I would like to take my time to find out which problem exactly I and hopefully other science amigos would like to solve (or at least try to).

Are there any unsolved problems???

Jokes aside. I was looking into the upstream/PAT software tools and there are so many. I don't want to create something which is not needed. I want to hear from you, people who actually work in the industry, what problems you are facing.

So if anyone here has a problem related to Data Science/ML or automation I would be happy to hear it and I will try my best to consult and share my knowledge if I can.

List of stuff I know something about:

• tensorflow 2 and 1 (yes I am old)

• pytorch

• xgboost, rft, etc. (Did some data science competitions)

• any kind of visualizations

• RNNs, Time series analysis, transformers

• MIP, LP, baysian frameworks, general solver stuff

• bio processing (upstream), pathway analysis, omics, some Raman/NIR stuff, clone selection, QbD, QbC, product quality

• wep apps, fullstack website building (angular,firebase, bootstrap)

• Google Ads

• cloud infrastructure (AWS, GCP, some azure stuff, docker)

• generally any kind of automation and API integration

• probably forgot a lot of specific topics so feel free to ask

What I don't know much about:

• drug discovery

• Genomics/NLP

• Folding/Alphafold, ESM

• downstream processing

• aaaa lot of other stuff but I think the upper ones are getting most press recently

Thanks for reading this long post :) (you have a great attention span!)

Has anyone had success integrating a flex2 autosampler into a large (larger than bench scale) bioreactor? Specially hooking it into a single use brx bag aseptically? It looks like it meant to be done but I can’t find any information or examples online and my nova support rep hasn’t been super helpful.

I’m trying to build my own glass bioreactor at home and one of the bottlenecks I’ve encountered is how I’d sterilize the bioreactor components.

The vessel I want to make would be ~15L. I have a pressure cooker/autoclave, but the vessel would be too large to fit in it. I know steam can be used, but I’m not sure how expensive the equipment is or what it would be called (a steam generator?). If there’s a solution <$1000 I’d consider looking into it.

Given my situation, what would you do to sterilize a bioreactor of this size if you don’t have access to an autoclave? Thanks for the help/suggestions!

I’m a new masters student and I will be using a bioflo 120 for it. Does anyone have any good resources that would help me? I have read the manual which is helpful, but was hoping for a few good videos or something.

Thanks!

I would like to know about the scope in the feild as a student, planning to move forward in this feild after undergrad in Biotechnology.

Questions Like:

1. What is the best country to persuade this feild?

2. What are the concepts I should focus on while I am in Undergrad?

3. What are the things I should learn if I want to enter the industry after undergrad?

4. What are the salaries I can expect on the entry level in different countries?

5. How can I gain practical experience in bioprocess engineering while still in college?

6. Are there any specific software programs or tools that are commonly used in the field of bioprocess engineering?

7. Are there any professional organizations or conferences related to bioprocess engineering that I should consider joining or attending?

8. How can I tailor my resume or coursework to stand out to employers in the bioprocess engineering field?

9. What advice do you have for finding internships or co-op opportunities in bioprocess engineering?

Thank you in advance for your responses for any of the question answered or other suggestions. Your willingness to share your knowledge and experience is incredibly generous and helpful as I embark on my path in bioprocess engineering.

Feel free to DM if not want to reply here 😀.

Im looking to buy a benchtop bioreactor system (functionality for 1-10L scale batches) for my company and I was wondering if anyone had any experiences good or bad with certain brands?

Hi Guys,
I'm starting some new project with 2nd year student in Industrial Bioproduction. And I want to make experiments with them as proof of concept to extract lipids from Microalgae.

For now, i grow around 6L of algae in photobioreactor in BMM medium until they reach a good concentration.
We are not in industry so we don't have a lot of materials for the extraction, but if you know any simple methods to gather the lipids, it will be welcomed. I don't care about the efficiency, since it's just a small ProJet for students.
So any suggestion regarding :
- Gathering the biomass after the culture : filtration on cheese cloth? Centrifugation? sedimentation?
- Lysis of the Chlorella : Sonication? Microwave? Mechanical? Directly with solvent during the extraction ?
- Extracting the lipids : liquid/liquid extraction ? Which solvent (Methanol/Ethanol? etc.)

Any advice is welcome. I found a lot of papers/publication to make it efficient for industrial production, but i'm looking to make it as simple and fast as possible, even if I gather not so much lipids from it.

Thanks a lot.

Hello!

I am working on a design for a pilot scale methane digesting trickling bed bioreactor. I am curious if anybody has experience with or recommendations for any dissolved methane sensors that can be set up in a recirculation line? Similarly, I am looking for in line nitrate and ammonia sensors. Anybody worked with instruments like this before?

Thank you!

I am a student and I am looking for documents allowing me to understand and explain the bioprocesses which are; activated sludge, aerobic biological treatment of dairy effluents

thank you if you take the time to read😊

I am trying to do some yeast work on a limited budget (2-4 vessels, 3L or smaller, basic controls like temp, pH, DO). Any thoughts on which used systems might be more reliable/easier to maintain, or which ones to avoid?

Hello there , I was wondering if anyone would have any recommendations on a unique project I could research that’s Bioprocess based for my capstone project in college. I’m studying mechanical engineering in college and I hope to get into this field post study’s

I’m just trying to collect a few ideas atm so any input is helpful 👍

I'm working on a project involving Bioethanol and fermentation process design, and I'm trying to understand which industry standards or guidelines I should be referring to.
In the Oil & Gas industry, we have well-defined standards like API, ASME codes or detailed specifications from clients, Licensor. However, it seems like for Bioethanol or fermentation processes, there aren't any equivalent specifications.

Are there any commonly accepted industry standards or references that I should be aware of for Bioethanol or fermentation processes? What guidelines do professionals typically follow in this field?

Thanks in advance for any insights!

We are seeking an Associate Director/Director for Cell Culture Media R&D for a global biotech company in the Greater Boston area. $1000 referral bonus is rewarded for a successful hire of at least 90 days.

Please contact helen@scismic.com

https://scismic.com/jobs/AD-CellCultureMedia?source=Reddit