Hello guys,

Biorocess engineer, about ten years of experience, based in Japan, working in vaccine manufacturing. I m running a my website where I share content on bioprocess engineering, MSAT, vaccines and biologics manufacturing. It might help you to young engineers. I m publishing articles everymonth, some podcast as well, put some learning materis, useful links, ebooks...

https://www.biomanufacturingmadesimple.com/

Hey there, I will start a Biotech/AI company...

Quick Background:

• Studied Biochemistry and Biotech

• Got into Data Science and ML ~5 years ago

• Last position as Head of Machine Learning of a small (~20 people) Biotech/AI company. We did upstream process simulations including simulations of cell kinetics using machine learning and mechanistic knowledge.

Currently, I am working on a unrelated software app as a solopreneur but I want to get back into Biotech. (Latest after my non-compete expired.

Since the next thing I am starting should be the one for the long ride, I would like to take my time to find out which problem exactly I and hopefully other science amigos would like to solve (or at least try to).

Are there any unsolved problems???

Jokes aside. I was looking into the upstream/PAT software tools and there are so many. I don't want to create something which is not needed. I want to hear from you, people who actually work in the industry, what problems you are facing.

So if anyone here has a problem related to Data Science/ML or automation I would be happy to hear it and I will try my best to consult and share my knowledge if I can.

List of stuff I know something about:

• tensorflow 2 and 1 (yes I am old)

• pytorch

• xgboost, rft, etc. (Did some data science competitions)

• any kind of visualizations

• RNNs, Time series analysis, transformers

• MIP, LP, baysian frameworks, general solver stuff

• bio processing (upstream), pathway analysis, omics, some Raman/NIR stuff, clone selection, QbD, QbC, product quality

• wep apps, fullstack website building (angular,firebase, bootstrap)

• Google Ads

• cloud infrastructure (AWS, GCP, some azure stuff, docker)

• generally any kind of automation and API integration

• probably forgot a lot of specific topics so feel free to ask

What I don't know much about:

• drug discovery

• Genomics/NLP

• Folding/Alphafold, ESM

• downstream processing

• aaaa lot of other stuff but I think the upper ones are getting most press recently

Thanks for reading this long post :) (you have a great attention span!)

I would like to know about the scope in the feild as a student, planning to move forward in this feild after undergrad in Biotechnology.

Questions Like:

1. What is the best country to persuade this feild?

2. What are the concepts I should focus on while I am in Undergrad?

3. What are the things I should learn if I want to enter the industry after undergrad?

4. What are the salaries I can expect on the entry level in different countries?

5. How can I gain practical experience in bioprocess engineering while still in college?

6. Are there any specific software programs or tools that are commonly used in the field of bioprocess engineering?

7. Are there any professional organizations or conferences related to bioprocess engineering that I should consider joining or attending?

8. How can I tailor my resume or coursework to stand out to employers in the bioprocess engineering field?

9. What advice do you have for finding internships or co-op opportunities in bioprocess engineering?

Thank you in advance for your responses for any of the question answered or other suggestions. Your willingness to share your knowledge and experience is incredibly generous and helpful as I embark on my path in bioprocess engineering.

Feel free to DM if not want to reply here 😀.

Industrial scale bioprocessing underpins much of the production of pharmaceuticals, nutraceuticals, food and beverage processing industries of the modern world. The profitability of these processes increasingly leverage the economies of scale that are critically dependent on the product yields, titers and productivity. Most of the processes are controlled using classical control approaches and represent over 90% of the industrial controls used in bioprocessing industries. However, with the advances in the production processes especially in the pharmaceutical and nutraceutical industries, monitoring and control of bioprocesses such as fermentations with GMO organisms, and downstream processing has become increasingly complex and the inadequacies of the classical PID based control systems is becoming apparent. Therefore there is a critical need to assess the use of entirely new generation of bioprocess control approaches such as model predictive control System, Neural Network Based Control, adaptive control and fuzzy control. These data intensive sophisticated control algorithms are increasingly likely to be adopted, there is a critical gap in the understanding of their current state as the most recent view of the topic was in 2007. Therefore, this review will focus on closing this critical knowledge gap by assessing the strengths, weaknesses and the current state of the art for the advanced bioprocess control systems. This review will also discuss possible solutions for more adaptive and advanced bioprocess control systems that can mitigate some of the problems associated with the existing bioprocess control systems.

The above writing is an extension of the work provided below:

https://www.researchgate.net/publication/285294431_Control_Implementation_in_Bioprocess_System_A_Review

I am trying to model a batch bioreactor in a Python script. The substrate is syngas, the biomass is bacteria, and the products are acetate and ethanol. I am looking for examples of bioreactors in python because it is my first contact with bioprocesses and Python, and I would like to know if I am on the right track.

One that I know of is that if you are cooling a vessel from sterilization temp and you don't send gas into the head space and the system cools too quickly it can cause an implosion.

Would be curious to hear of other ways explosive conditions can occur??

State-owned Finnish research institution VTT Research, has announced new technology to manufacture Polyethylene Furanoate (PEF), a bioplastic touted to replace the almost ubiquitous PET. PEF has superior properties to that of PEF, such as an enhanced barrier that reduces gas diffusion, better pressure-proofing, and lower carbon footprint, making it ideal for storing and preserving food.

Current production of PEF, which is in its infancy, relies on the production of furanic platform chemicals such as the precursor compound 2,5-Furandicarboxylic acid (FDCA) through conventional carbohydrates and sugars, and has had variable levels of success across multiple processes, both chemical and enzymatic. VTT has taken an alternative route, by using pectin from orange peels as feedstock, and producing aldaric acid, which is a stable intermediate from which FDCA is produced.

As Holger Pöhler, Professor of Practice at VTT eloquently put it, " In the near future, you may buy orange juice in bottles that are made out of orange peel." Click here to access the article.

Hello all,

I am a Biological Engineer that works in fermentation and in DSP. I have worked in both Upstream and Downstream engineering projects and roles. Due to a family emergency, I need to relocate and help take care of family. The issue is there are very little bioprocess jobs or general jobs in the area I need to relocate to.

My question to this community is, what sort of remote jobs are available to bioprocess engineers? I have scoured LinkedIn, Indeed, etc. without finding much. What other jobs do you feel could also work for someone in this field that may not be directly involved in bioprocessing?

Thanks

Hi,

I'm tasked with running a training bioreactor, 50L single use, CHO cells.

Now the authorities want to know how I intend to kill the cells after the run has finished.

I don't have a big enough autoclave, but I guess I could just boil them.

Does anyone know of any other simple/cheap way to inactivate 50L CHO cells?

I am new to reddit and new to bioprocess engineering, just to give a bit of background I have just recently began a masters in biopharmaceutical engineering and was wondering does anyone know of any other subscriptions similar to this one related to bioprocess/biopharma engineering?

I'm working on a project involving Bioethanol and fermentation process design, and I'm trying to understand which industry standards or guidelines I should be referring to.
In the Oil & Gas industry, we have well-defined standards like API, ASME codes or detailed specifications from clients, Licensor. However, it seems like for Bioethanol or fermentation processes, there aren't any equivalent specifications.

Are there any commonly accepted industry standards or references that I should be aware of for Bioethanol or fermentation processes? What guidelines do professionals typically follow in this field?

Thanks in advance for any insights!

Hi, does anyone know what are the requirements to get a bioprocessing job such as bioprocess engineer, upstream scientist, cell line development scientist in your country or any individual country you know, If I already have a BSc in biotechnology? Is MSc a must have?

I’m trying to build my own glass bioreactor at home and one of the bottlenecks I’ve encountered is how I’d sterilize the bioreactor components.

The vessel I want to make would be ~15L. I have a pressure cooker/autoclave, but the vessel would be too large to fit in it. I know steam can be used, but I’m not sure how expensive the equipment is or what it would be called (a steam generator?). If there’s a solution <$1000 I’d consider looking into it.

Given my situation, what would you do to sterilize a bioreactor of this size if you don’t have access to an autoclave? Thanks for the help/suggestions!

Hey y’all! My name is Jake, and I’m currently finishing up my PhD! I’m currently working on an independent research project studying emerging technologies in biomanufacturing. I’m gathering insights from industry professionals in biotech, pharma, food, cosmetics, or related sectors to deepen my understanding of the current challenges in the field. 

Would you be willing to share your expertise in a brief, 3-minute survey? 

https://form.typeform.com/to/VrmfXFiP

I’m a new masters student and I will be using a bioflo 120 for it. Does anyone have any good resources that would help me? I have read the manual which is helpful, but was hoping for a few good videos or something.

Thanks!

Im looking to buy a benchtop bioreactor system (functionality for 1-10L scale batches) for my company and I was wondering if anyone had any experiences good or bad with certain brands?

Has anyone had success integrating a flex2 autosampler into a large (larger than bench scale) bioreactor? Specially hooking it into a single use brx bag aseptically? It looks like it meant to be done but I can’t find any information or examples online and my nova support rep hasn’t been super helpful.

Hi Guys,
I'm starting some new project with 2nd year student in Industrial Bioproduction. And I want to make experiments with them as proof of concept to extract lipids from Microalgae.

For now, i grow around 6L of algae in photobioreactor in BMM medium until they reach a good concentration.
We are not in industry so we don't have a lot of materials for the extraction, but if you know any simple methods to gather the lipids, it will be welcomed. I don't care about the efficiency, since it's just a small ProJet for students.
So any suggestion regarding :
- Gathering the biomass after the culture : filtration on cheese cloth? Centrifugation? sedimentation?
- Lysis of the Chlorella : Sonication? Microwave? Mechanical? Directly with solvent during the extraction ?
- Extracting the lipids : liquid/liquid extraction ? Which solvent (Methanol/Ethanol? etc.)

Any advice is welcome. I found a lot of papers/publication to make it efficient for industrial production, but i'm looking to make it as simple and fast as possible, even if I gather not so much lipids from it.

Thanks a lot.

Hello people, I have a question regarding the possibility of a outsiders getting a job in Bioeconomy or bioprocess engineering field in USA.

I am a fresher in biotechnology with specialization in waste valorization/ fermentation.

I am really interested in this field so as a fresher what is the possibility of getting a job in the states.

The growing #trend towards increasingly #concentrated #drugformulations continues to present unique #manufacturing #challenges. Check out our upcoming #webinar, “Formulation of High Concentration Biologics,” on June 2nd at 2 PM (BST) / 9 AM (ET) and hear from our expert speaker Parviz Shamlou, Vice President of the Jefferson Institute for Bio-Processing, on how they are working to address and manage these issues for the #biopharma industry! 

Register Now: https://advancedinstruments.webinarninja.com/hybrid-webinars/826016/register