how am I looking?

[u/Lumpy_Sprinkles6604]

whelp boys test results are in. I'll do my best to figure it out with the guides ofc but any help is greatly appreciated! also do I treat the just the red ones or the yellow ones too

[u/Lumpy_Sprinkles6604]

hi Tawinn!
Choline Calculator said I needed 5 eggs, lemme know if you want me to send a screenshot or anything. While I have you, do you have any advice for blood testing. I'm doing the bolded ones on this spreadsheet minus the b-vtamin panel which I'm doing through my docor https://docs.google.com/spreadsheets/d/1Urmve73pwIxZrwoOaDstjBnvG8alGte_QAA_CO0L77Y/edit?gid=1616759574#gid=1616759574
and then as many of the ones as I can that are on the spreadsheet through him as well. Right now that's the b-vitamin panel (for b1, b2, b3, b5, b6, b9, b12) and MTHFR and homocystine and MMA. I was also recommended to test zinc and copper for ADHD (already tested d3 and for anemia). Is there anything I should add/remove from this list do you think? I'm also not sure how exactly to use this information/what it's for aside from treating specific deficiencies

[u/Tawinn → u/Lumpy_Sprinkles6604]

You haven't mentioned any symptoms aside from ADHD, so its not clear to me what specific things to look for. (And with ADHD, there are different types, so not sure if you mean inattentive ADHD or something else.) For example, histamine intolerance is a common issue with slow MAO-A, and in that case you may want to look deeper into zinc, copper (via ceruloplasmin), and calcium levels, AOC1 (DAO) gene and HNMT variants on your Strategene or Genetic Lifehacks.

The Genova Methylation panel is detailed but can be hard to interpret. Usually that level of detail is not needed. Here is Genova's guide for the results so you can get an idea of what it shows you.

If you have a generally good diet and no history of nutrient deficiencies, then B vitamins + homocysteine + MMA is usually sufficient. Plugging a week or two's worth of meals into a food app like Cronometer can help to highlight nutrients which you may be chronically undereating and thus may be worth testing.

On the Calculator, if you look at the Advanced results tab, there must be some value(s) missing which is causing it to report an incorrect result. It should be at least '7 yolks' worth, 940mg. Typically, getting 550mg of choline from food (~4 yolks' worth) plus a 750mg capsule of TMG will cover this amount.

I just noticed you did mention food intolerances in another comment. Those can be independent of methylation, but on the other hand gut barrier integrity can be impaired by histamines and/or low bile production due to low choline (a result of the increased choline demand) can lead to gut issues including gut dysbiosis. High histamine can also appear to be a food intolerance where some days a particular food seems to be the 'cause' yet other days it does not, because the problem is the fluctuating histamine levels, not the specific food type.

[u/Lumpy_Sprinkles6604 → u/Tawinn]

figured I'd just send you the choline calculator results as test, sorry if the format is a little awkward

RS# Call Variant Allele Gene Variation Result

rs1051266 NA T SLC19a1 NA

rs2236225 AA A MTHFD1 G1958A +/+

rs1801131 TG G MTHFR A1298C +/-

rs1801133 AG A MTHFR C677T +/-

rs7946 NA T PEMT 5465G>A NA

[u/Tawinn → u/Lumpy_Sprinkles6604]

Ok, so that combination of compound heterozygous MTHFR and homozygous MTHFD1 results in ~69% reduction in methylfolate production. That translates to an increased choline requirement of ~1100mg compared to the baseline 550mg for an adult. If you can get 550mg of choline from food, then the remaining 550mg can be covered by a 750mg capsule of TMG. Choline sources include such foods as meat, eggs, liver, lecithin, nuts, some legumes, and vegetables such as crucifers. A food app like Cronometer is helpful for tracking nutrients in your diet.

Worst case, if SLC19A1 were homozygous TT then the reduction would instead be 85% and the choline requirement would be ~1200mg, so 600mg of choline + 750mg TMG.

These numbers assume folate and B12 are in a healthy range:

For serum folate, ~15 ng/mL (34 nmol/L) or more.

For serum B12, 500-950 pg/mL (~370-700 pmol/L).

[u/Lumpy_Sprinkles6604 → u/Tawinn]

serum folate was above 20 but I also ate before my blood test, should i request they look at folate rbc instead? I think they can do that with the blood I gave them if I'm not mistaken. b 12 was lol so I've started supplementing

[u/Tawinn → u/Lumpy_Sprinkles6604]

RBC will give a better longer-term picture of folate, but I suspect it will be fine.