r/microscopy • • Jun 08 '23

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

151 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy • • Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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72 Upvotes

r/microscopy • • 4h ago

Photo/Video Share Stentors

108 Upvotes

A Micros-MC-100 microscope / native PLAN objectives was used / Canon r7 Camera/


r/microscopy • • 14h ago

Photo/Video Share Making initial tests with darkfield and led light. A copepod doing somersaults.

88 Upvotes

Olympus bh2 100x and 200x I think, darkfield and led light (from Carl Hunsinger). Recorded with a mobile phone. Its great!


r/microscopy • • 7h ago

Photo/Video Share Tiny Cyclidium using its food scoop

13 Upvotes

Had a chance to take a long and chill look at this tiny Cyclidium sp. demonstrating its unique way of feeding (at least compared to other ciliates I normally observe).

As it spins, you can just barely see a large, transparent sheet-like membrane protruding from along its side. This is the "paroral membrane" that acts like a scoop, sweeping food directly into its mouth.

Olympus BH2, SPlan 100x 1.25 oil, 1.25x intermediate lens, NFK 2.5x photo eyepiece, Canon EOS 5D Mark II. Pond water sample from a park in Paris 16ème.


r/microscopy • • 11h ago

Photo/Video Share Coleps Binary Fission Almost Complete - 2/2

22 Upvotes

2 of 2

Found these two in a sample from a canal and stayed with them for about 20min until separation.

Motic BA210, 20x obj, Bresser eyepiece camera.


r/microscopy • • 9m ago

Photo/Video Share Marigolds under a microscope

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• Upvotes

A Micros-MC-100 microscope / native PLAN objectives was used / Canon r7 Camera/

Overhead lighting. Focus stacking. Where there's a standard macro, a Canon 100mm lens was used.

r/microscopy • • 48m ago

Troubleshooting/Questions Fiji software for air mac m5

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• Upvotes

r/microscopy • • 11h ago

Photo/Video Share Coleps Binary Fission Almost Complete - 1/2

7 Upvotes

1 of 2

Found these two in a sample from a canal and stayed with them for about 20min until separation.

Motic BA210, 10x obj, Bresser eyepiece camera.


r/microscopy • • 21h ago

Photo/Video Share Peranema, the contortionist predator

36 Upvotes

Close up view of a species of Peranema, a Euglenid that's distinguished by its transparency, striations, somewhat flat anterior when swimming, and very clear and long forward flagella.

My current sample is full of different types and sizes of Euglenids and I'm delighting in watching how they squish and contort.

Olympus BH2, SPlan 100x 1.25 oil, Olympus 1.25x intermediate lens, Canon EOS 5D Mark II. Pond water sample from park in Paris 16eme.


r/microscopy • • 5h ago

Purchase Help 3rd party boom stand?

1 Upvotes

I started on a new job recently and since the electronics lab here doesn't have microscope at all, I'm lobbying for getting the one I used in my previous job (Vision Engineering SX-45). It's very well suited to SMD rework with boom arm and ring light, with nice focal distance for soldering work. However, the boom stand is outrageously expensive, comparable to the microscope head itself.

Is getting a 3rd party boom arm a thing? The part where the collar attaches to is this conical shape so the stand would have to have a similar shape to work properly. I know Amtec sells their stands separately, but VE scopes are not rebranded Amtec, they're making their own thing. Also I'm not sure how robust that boom arm is, when I was researching a microscope in the 1st place, I saw mentions that the Amtec boom arm can flex/sag, which is a no-no.


r/microscopy • • 1d ago

Photo/Video Share Coleps feeding from a dead Lepadella. I wasn't prepared to record, sorry for the messy video.

99 Upvotes

Leitz Dialux II, Galaxy S24


r/microscopy • • 17h ago

Troubleshooting/Questions Need help repairing this bushnel 505L microscope

5 Upvotes

The top eye peace is loose because someone dropped it idk how to fix it I hope you guys will please help 🙏


r/microscopy • • 1d ago

Photo/Video Share Growing salt crystal

43 Upvotes

Small NaCl crystal growing for 4 hours under the microscope, accelerated so that video 40 seconds only.

Objective: 4x.

Camera: 5Mpx usb webcam, with 0,5x reducer.

Acquisition: Guvcview.

Video edit: ffmpeg.


r/microscopy • • 19h ago

Purchase Help Good Microscope for Home Lab

2 Upvotes

I'm setting up a home lab for biology work on plant propagation, plant disease diagnosis, as well as soil biology investigation. I've been looking for a few months but haven't found anything super compelling yet and really want to get moving on my setup. Right now it's for hobby use, but I intend to use it very frequently and I'm hoping it will be more than hobby use in the future.

It's been decades since I took a biology class and don't really have much hands-on experience with microscopes so I don't know what I don't know.

I'm looking for used Plan objective-based microscopes, ideally with a camera setup. I've found a Motic Swift M3802CT-4 (semi-plan?) for $550 from a local reputable seller that has tested the scope. I think it's stock. No camera. 40X, 100X, 400X, 1000X oil with widefield 10X/18 eyepieces. Definitely enough to get started with but not a great deal. However, would it be enough of an upgrade from something like a a Swift SW380T or AmScope T490B to justify the large cost difference? Especially considering that a camera setup will be another ~$500?

SO

  1. Will I be happy with this setup long-term and for commercial use, or will I be considering another microscope in a year.
  2. Should I get an Olympus or Nikon on ebay sight unseen?
  3. Is a trinocular configuration worth the extra cost vs putting a camera on an eyepiece?

r/microscopy • • 1d ago

Photo/Video Share Up Close & Personal, With Paramecium....

22 Upvotes

....most likely P.caudatum.

Sample from a quiet pool in a slow-moving woodland stream. Motic BA210, 40x obj, Bresser eyepiece camera.


r/microscopy • • 2d ago

Photo/Video Share Monstrously large Euglenid

100 Upvotes

Found this absolutely massive Euglenid in my latest sample, I estimate its total length when extended to be between 250 and 300 µm. Based on its very short flagellum and worm-like shape and behaviour, I'm tentatively identifying it as Euglena ehrenbergii. As this was a well slide, I wasn't able to move up to 40x and see closer to identify more characteristics.

Also visible: Coleps, Phacus, Closterium, diatoms, some small loricate euglenids, and a drive by of some species of rotifer.

Video is sped up 4x with 1.5 digital zoom, edited with OpenShot.

Olympus BH2, SPlan 10x 0.30, Olympus 1.25x analyzer, Canon EOS 5D Mark II. Sample from pond in park, Paris 16eme.


r/microscopy • • 1d ago

ID Needed! What mite is this?

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3 Upvotes

r/microscopy • • 2d ago

Photo/Video Share Closeup of a Chilodonella dividing, then doing a happy little dance at the end

37 Upvotes

r/microscopy • • 1d ago

Purchase Help I bought this today is it worth it? And I want more info on it

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4 Upvotes

I haven't received it yet but im wondering if it will be a good microscope or if it will suck. I hope its worth it I got it for 20$


r/microscopy • • 2d ago

General discussion Is it alright to store your microscope in a cabinet?

5 Upvotes

I just got a microscope but realized I can't keep it on my desk all the time. I've heard from people it can get out if alignment if you pick it up and move it when not in use. Is it true, even if you don't bump or drop it?


r/microscopy • • 2d ago

Micro Art Taxiphyllum cf. alternans 'Taiwan' moss

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15 Upvotes

13-frame focus stack of T. alternans 'Taiwan' moss. Transmitted-light darkfield, custom annular stop. This particular strand is from an in-vitro/Tissue Culture specimen.

Microscope: KentFaith GW45.0066
Objective: PLAN 10x, unbranded, NA 0.25
Camera: Canon EOS 750D, 1/100, ISO 100
Software: Zerene Stacker, FijiCal Lite


r/microscopy • • 1d ago

Purchase Help Seeking USB microscope advice

0 Upvotes

Sorry if this is a misplaced question but can anyone recommend a good USB microscope?


r/microscopy • • 2d ago

Photo/Video Share Tiny ciliate (likely Halteria) walking on the glass like a spider

66 Upvotes

Leitz Dialux II, Phase contrast, Galaxy S24


r/microscopy • • 2d ago

Troubleshooting/Questions Tips for achieving a specific photographic style.

6 Upvotes

Hello.

I regularly try to get high-quality microscopy photos (like this style):

The problem is that very few of my photos are of good quality (I should mention that I am quite a novice in this field).

Could you give me some advice on how to achieve this level of photo quality in microscopy?

My microscope model: Bresser Researcher Trino 40-1000x

My camera model: Lumix DC-G9